Coding

Part:BBa_K3173013

Designed by: Thorge Dobbertin   Group: iGEM19_Ruperto_Carola   (2019-10-16)


cam-TA

Camphor based Transcription control (Tet analogous). Binds to BBa_K3173015 to activate transcription in absence of camphor.
As described by: Ikushima S, Zhao Y, Boeke JD. Development of a Tightly Controlled Off Switch for Saccharomyces cerevisiae Regulated by Camphor, a Low-Cost Natural Product. G3 (Bethesda). 2015;5(10):1983–1990. Published 2015 Jul 22. doi:10.1534/g3.114.012765.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 466
    Illegal PstI site found at 602
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 602
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 466
    Illegal PstI site found at 602
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 466
    Illegal PstI site found at 602
    Illegal NgoMIV site found at 561
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None